bluecell

Acquire images in ratiometric and nonratiometric modes
Acquire images using one or two dye-sets. Either can be ratiometric or nonratiometric
Simultaneous control of excitation and emission wavelength for each dye-set
Live video mode for focusing
Shading correction (see image below)
ΔF/F0 for any nonratiometric wavelengths; F0 can be averaged to a low-noise image
Background subtraction
View and save a smaller acquisition area in the field of view to accelerate acquisition and save hard disk space
Store images to a ring buffer for the highest performance
Always-on-top Acquisition Control Panel for on-the-fly changes to acquisition parameters

Multiple image display
Generates ratio images, pseudocolor mapped based on ratio or intensity values
Annotates image files with comment tags and voice tags
Prints on-line graphs. Copy and paste graphs into other programs
Dragging a "sync" cursor along the time-axis of the graph will automatically display the image acquired
at the corresponding time (see below)

Switch acquisition rates at will when recording continuously
Allows episodic acquisition protocols, e.g. with electrophysiology
Precise timing control of camera, wavelength changers and shutter devices
Timing Overview window that illustrates device control and image acquisition timing, allowing
for precise calculation of fluorescence exposure cycles

Imaging Workbench 6 has enhanced features for cooperative, simultaneous imaging and electrophysiology
when used in conjunction with 8 through 10.
IW is also compatible with PatchMaster v2 from Heka.
Runs concurrently with Clampex or PatchMaster
Receives software trigger from Clampex to start image acquisition
Digital outputs are sent through the INDEC BioSystems DIO-3 interface box or equivalent. Digital inputs
to the DIO-3 can trigger image acquisition
Displays electrophysiology data, fluorescence intensities and/or ion concentrations in the same graph
A "sync" cursor can be placed anywhere on the electrophysiology data, and the image acquired
at the corresponding time will be automatically displayed

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